Cathepsin‐B Induced Controlled Release from Peptide‐Capped Mesoporous Silica Nanoparticles. Issue 47 (10th October 2014)

• Record Type: Journal Article
• Authors: de la Torre, Cristina; Mondragón, Laura; Coll, Carmen; Sancenón, Félix; Marcos, María D.; Martínez‐Máñez, Ramón; Amorós, Pedro; Pérez‐Payá, Enrique; Orzáez, Mar
• Is Part Of: Chemistry. Volume 20:Issue 47(2014)
• Page Start: 15309
• Page End: 15314
• Publication Date: 2014-10-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201404382
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>New capped silica mesoporous nanoparticles for intracellular controlled cargo release within cathepsin B expressing cells are described. Nanometric mesoporous MCM‐41 supports loaded with safranin O (<bold>S1‐P</bold>) or doxorubicin (<bold>S2‐P</bold>) containing a molecular gate based on a cathepsin B target peptidic sequence were synthesized. Solids were designed to show "zero delivery" and to display cargo release in the presence of cathepsin B enzyme, which selectively hydrolyzed in vitro the capping peptide sequence. Controlled delivery in HeLa, MEFs WT, and MEFs lacking cathepsin B cell lines were also tested. Release of safranin O and doxorubicin in these cells took place when cathepsin B was active or present. Cells treated with <bold>S2‐P</bold> showed a fall in cell viability due to nanoparticles internalization, cathepsin B hydrolysis of the capping peptide, and cytotoxic agent delivery, proving the possible use of these nanodevices as new therapeutic tools for cancer treatment.</p> </abstract>