Cell‐Penetrating and Neurotargeting Dendritic siRNA Nanostructures1. Issue 6 (17th December 2014)

• Record Type: Journal Article
• Authors: Brunner, Korbinian; Harder, Johannes; Halbach, Tobias; Willibald, Julian; Spada, Fabio; Gnerlich, Felix; Sparrer, Konstantin; Beil, Andreas; Möckl, Leonhard; Bräuchle, Christoph; Conzelmann, Karl‐Klaus; Carell, Thomas
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 6(2015)
• Page Start: 1946
• Page End: 1949
• Publication Date: 2014-12-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201409803
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We report the development of dendritic siRNA nanostructures that are able to penetrate even difficult to transfect cells such as neurons with the help of a special receptor ligand. The nanoparticles elicit strong siRNA responses, despite the dendritic structure. An siRNA dendrimer directed against the crucial rabies virus (RABV) nucleoprotein (N protein) and phosphoprotein (P protein) allowed the suppression of the virus titer in neurons below the detection limit. The cell‐penetrating siRNA dendrimers, which were assembled using click chemistry, open up new avenues toward finding novel molecules able to cure this deadly disease.</p> </abstract>