Na+, K+-ATPase and Ca2+-ATPase activities in basal and microvillous syncytiotrophoblast membranes from preeclamptic human term placenta. (February 2015)

• Record Type: Journal Article
• Authors: Abad, Cilia; Vallejos, Catalina; De Gregorio, Nicole; Díaz, Paula; Chiarello, Delia I.; Mendoza, Mariana; Piñero, Sandy; Proverbio, Teresa; Botana, Desirée; Rojas, Próspero; Riquelme, Gloria; Proverbio, Fulgencio; Marín, Reinaldo
• Is Part Of: Hypertension in pregnancy. Volume 34:Number 1(2015)
• Page Start: 65
• Page End: 79
• Publication Date: 2015-02
• Subjects: Hypertension in pregnancy -- Periodicals; Pregnancy -- Complications -- Periodicals; 618.3261
• DOI: 10.3109/10641955.2014.973038
• Languages: English
• ISSNs: 1064-1955

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Objective</italic>: The aim of this study is to evaluate the effect of preeclampsia on the level of lipid peroxidation, activity and expression of both plasma membrane Ca²⁺- and Na⁺, K⁺-ATPases in syncytiotrophoblast. <italic>Methods</italic>: The level of lipid peroxidation was estimated by measuring TBARS. ATPase activities were quantified by a colorimetric method measuring the amount of inorganic phosphate during the assay. Expression of Ca²⁺- and Na⁺, K⁺-ATPases in syncytiotrophoblast plasma membranes and term placenta tissue sections was investigated using Western blot and immunohistochemistry, respectively. <italic>Results</italic>: Our results show a higher level of lipid peroxidation of syncytiotrophoblast plasma membranes from preeclamptic, as compared to uncomplicated pregnant women. Preeclampsia also significantly reduced the activity of Ca²⁺- and Na⁺, K⁺-ATPases; however, expression of both ATPases was unaffected. <italic>Conclusion</italic>: Our findings suggest that the reduction of Ca²⁺- and Na⁺, K⁺-ATPase activities during preeclampsia could be at least partially due to an increased level of lipid peroxidation of the syncytiotrophoblast plasma membranes.</p> </abstract>