Alteration of culture fluid proteins by cadmium induction in Phanerochaete chrysosporium. (8th November 2013)

• Record Type: Journal Article
• Authors: Chen, Guiqiu; Zhou, Ying; Zeng, Guangming; Liu, Hongyu; Yan, Ming; Chen, Anwei; Guan, Song; Shang, Cui; Li, Huanke; He, Jianmin
• Is Part Of: Journal of basic microbiology. Volume 55:issue 2(2015:Feb.)
• Page Start: 141
• Page End: 147
• Publication Date: 2013-11-08
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201300398
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201300398-sec-0001" sec-type="section"> <p>Microorganisms need to resist the hazards posed by heavy metals during the process of metal adsorption. <italic>Phanerochaete chrysosporium</italic> is a fungus that is efficiently used for heavy‐metal biosorption in wastewater treatment. Extraction and analysis of proteins induced by heavy metals can help to understand the regulatory mechanisms of <italic>P. chrysosporium</italic> in wastewater treatment. In this study, <italic>P. chrysosporium</italic> was exposed to 50 μM cadmium nitrate. A maximum cadmium adsorption capability of 77.1 mg/g dry biomass was found after 65 h, which was accompanied by a relatively higher protein concentration. After separation of the culture fluid proteins by two‐dimensional difference gel electrophoresis (2D‐DIGE), three differentially expressed proteins were detected from 17 spots. By using 2D‐DIGE, followed by matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight mass spectrometry (MALDI‐TOF/TOF MS), glutathione <italic>S</italic>‐transferase, glyceraldehyde‐3‐phosphate dehydrogenase, and malate dehydrogenase were identified. All three enzymes play important roles in the oxidative stress caused by cadmium.</p> </sec> </abstract>