Chlamydia muridarum Infection Associated Host MicroRNAs in the Murine Genital Tract and Contribution to Generation of Host Immune Response. (28th June 2014)

• Record Type: Journal Article
• Authors: Gupta, Rishein; Arkatkar, Tanvi; Yu, Jieh‐Juen; Wali, Shradha; Haskins, William E.; Chambers, James P.; Murthy, Ashlesh K.; Bakar, Sazaly Abu; Guentzel, M Neal; Arulanandam, Bernard P.
• Is Part Of: American journal of reproductive immunology. Volume 73:Number 2(2015:Feb.)
• Page Start: 126
• Page End: 140
• Publication Date: 2014-06-28
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12281
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12281-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12281-sec-0001" sec-type="section"> <title>Problem</title> <p> <italic>Chlamydia trachomatis</italic> (CT) is the leading sexually transmitted bacterial infection in humans and is associated with reproductive tract damage. However, little is known about the involvement and regulation of microRNAs (miRs) in genital CT.</p> </sec> <sec id="aji12281-sec-0002" sec-type="section"> <title>Methods</title> <p>We analyzed miRs in the genital tract (GT) following <italic>C. muridarum</italic> (murine strain of CT) challenge of wild type (WT) and CD4<sup>+</sup> T‐cell deficient (CD4<sup>−/−</sup>) C57BL/6 mice at days 6 and 12 post‐challenge.</p> </sec> <sec id="aji12281-sec-0003" sec-type="section"> <title>Results</title> <p>At day 6, miRs significantly downregulated in the lower GT were miR‐125b‐5p, ‐16, ‐214, ‐23b, ‐135a, ‐182, ‐183, ‐30c, and ‐30e while ‐146 and ‐451 were significantly upregulated, profiles not exhibited at day 12 post‐bacterial challenge. Significant differences in miR‐125b‐5p (+5.06‐fold change), ‐135a (+4.9), ‐183 (+7.9), and ‐182 (+3.2) were observed in <italic>C. muridarum</italic>‐infected CD4<sup>−/−</sup> compared to WT mice. <italic>In silico</italic> prediction and mass spectrometry revealed regulation of miR‐135a and ‐182 and associated proteins, <italic>that is</italic>, heat‐shock protein B1 and alpha‐2HS‐glycoprotein.</p> </sec> <sec id="aji12281-sec-0004" sec-type="section"> <title>Conclusion</title> <p>This study provides evidence on regulation of miRs following genital chlamydial infection suggesting a role in pathogenesis and host immunity.</p> </sec> </abstract>