Comparison of reversed‐phase liquid chromatography and hydrophilic interaction chromatography for the fingerprint analysis of Radix isatidis2. Issue 9 (10th April 2014)

• Record Type: Journal Article
• Authors: Zhang, Cong‐En; Xiong, Yin; Dong, Qin; Gao, Dan; Zhang, Le‐Le; Ma, Li‐Na; Peng, Cheng; Dong, Xiao‐Ping; Yan, Dan
• Is Part Of: Journal of separation science. Volume 37:Issue 9/10(2014)
• Page Start: 1141
• Page End: 1147
• Publication Date: 2014-04-10
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201301159
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Radix isatidis</italic> is a famous anti‐influenza virus herbal medicine traditionally taken as a water decoction. However, the chemical fingerprint analysis of <italic>Radix isatidis</italic> is dominantly based on RPLC, from which it is difficult to obtain fingerprint information of hydrophilic compounds. Here, we developed the separation of <italic>Radix isatidis</italic> by RPLC and hydrophilic interaction chromatography, comparing the traditional RPLC fingerprint with the hydrophilic interaction chromatography fingerprint. Besides, an anti‐viral assay of <italic>Radix isatidis</italic> was conducted to evaluate its efficacy. The fingerprint–efficacy relationships between the fingerprints and the anti‐viral activity were further investigated with principal component regression analysis. The results showed that the anti‐viral activity correlated better with the hydrophilic interaction chromatography fingerprint than with the RPLC fingerprint. This study indicates that hydrophilic interaction chromatography could not only be a complementary method to increase the fingerprint coverage of conventional RPLC fingerprint, but also can better represent the efficacy and quality of <italic>Radix isatidis</italic>.</p> </abstract>