Neurochemistry in shiverer mouse depicted on MR spectroscopy. Issue 6 (14th November 2013)

• Record Type: Journal Article
• Authors: Takanashi, Jun‐ichi; Nitta, Nobuhiro; Iwasaki, Nobuaki; Saito, Shigeyoshi; Tanaka, Ryuta; Barkovich, A. James; Aoki, Ichio
• Is Part Of: Journal of magnetic resonance imaging. Volume 39:Issue 6(2014)
• Page Start: 1550
• Page End: 1557
• Publication Date: 2013-11-14
• Subjects: Magnetic resonance imaging -- Periodicals; 616
• DOI: 10.1002/jmri.24306
• Languages: English
• ISSNs: 1053-1807

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jmri24306-sec-0001" sec-type="section"> <title>Purpose</title> <p>To evaluate the neurochemical changes associated with hypomyelination, especially to clarify whether increased total <italic>N</italic>‐acetylaspartate (tNAA) with decreased choline (Cho) observed in the thalamus of <italic>msd</italic> mice with the <italic>plp1</italic> mutation is a common finding for hypomyelinating disorders.</p> </sec> <sec id="jmri24306-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>We performed magnetic resonance imaging (MRI) and proton MR spectroscopy (<sup>1</sup>H‐MRS) of the thalamus and cortex of postnatal 12‐week shiverer mice devoid of myelin basic protein (mbp), heterozygous and wild‐type mice with a 7.0T magnet. Luxol Fast Blue staining and immunohistochemical analysis with anti‐Mbp, Gfap, Olig2, and NeuN antibodies were also performed.</p> </sec> <sec id="jmri24306-sec-0003" sec-type="section"> <title>Results</title> <p>In the thalamus, decreased Cho and normal tNAA were observed in shiverer mice. In the cortex, tNAA, Cho, and glutamate were decreased in shiverer mice. Histological and immunohistochemical analysis of shiverer mice brains revealed hypomyelination in the thalamus, white matter, and cortex; astrogliosis and an increased number of total oligodendrocytes in the white matter; and a decreased number of neurons in the cortex.</p> </sec> <sec id="jmri24306-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The reduction of Cho on <sup>1</sup>H‐MRS might be a common marker for hypomyelinating disorders. A normal tNAA level in the thalamus of shiverer mice might be explained by the presence of mature oligodendrocytes, which enable neuron‐to‐oligodendrocyte NAA transport or NAA catabolism. <bold>J. Magn. Reson. Imaging 2014;39:1550–1557</bold>. © <bold>2013 Wiley Periodicals, Inc</bold>.</p> </sec> </abstract>