Improved conditions for periodate/Schiff's base‐based fluorescent staining of glycoproteins with dansylhydrazine in SDS‐PAGE. Issue 10 (10th April 2014)

• Record Type: Journal Article
• Authors: Zhou, Xuan; Hong, Guo‐Ying; Huang, Bin‐Bin; Duan, Yuan‐Meng; Shen, Jia‐Yi; Ni, Mao‐Wei; Cong, Wei‐Tao; Jin, Li‐Tai
• Is Part Of: Electrophoresis. Volume 35:Issue 10(2014:May)
• Page Start: 1439
• Page End: 1447
• Publication Date: 2014-04-10
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201300595
• Languages: English
• ISSNs: 0173-0835

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>An improved periodate/Schiff's base based fluorescent stain with dansylhydrazine (DH) for glycoproteins in 1D and 2D SDS‐PAGE was described. Down to 4–8 ng of glycoproteins can be selectively detected within 2 h, which is approximately 16‐fold higher than that of original protocol, but similar to that of Pro‐Q Emerald 488 stain (Invitrogen, Carlsbad, USA). Furthermore, subsequent study of deglycosylation, glycoprotein affinity isolation, and LC‐MS/MS analysis were performed to confirm the specificity of the improved method. As a result, improved DH stain may provide a new choice for selective, economic, MS compatible, and convenient visualization of gel‐separated glycoproteins.</p> </abstract>