Autologous albumin enhances the humoral immune response to capsular polysaccharide covalently coattached to bacteria‐sized latex beads. Issue 5 (27th February 2014)

• Record Type: Journal Article
• Authors: Colino, Jesus; Duke, Leah; Snapper, Clifford M.
• Is Part Of: European journal of immunology. Volume 44:Issue 5(2014:May)
• Page Start: 1433
• Page End: 1443
• Publication Date: 2014-02-27
• Subjects: Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201344266
• Languages: English
• ISSNs: 0014-2980

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Abundant autologous proteins, like serum albumin, should be immunologically inert. However, individuals with no apparent predisposition to autoimmune disease can develop immune responses to autologous therapeutic proteins. Protein aggregation is a potential major trigger of these responses. Adsorption of proteins to particles provides macromolecular size and may generate structural changes in the protein, resembling aggregation. Using aldehyde/sulfate latex beads coated with murine serum albumin (MSA), we found that BALB/c mice mounted MSA‐specific IgG responses that were dependent on CD4<sup>+</sup> T cells. IgGs were specific for MSA adsorbed to solid surfaces and noncross‐reactive with human, bovine, or pig albumins. T cells induced in response to MSA augmented the primary and induced boosted secondary IgG and IgM responses specific for the T cell‐independent antigen, capsular polysaccharide of <italic>Streptococcus pneumoniae</italic> type 14 (PPS14), when the latter was attached to the same bead. Similar to the anti‐MSA IgG response, the boosted PPS14‐specific IgG secondary response was CD4<sup>+</sup> T‐cell dependent, displayed a typical carrier effect, and was enhanced by, but did not require, Toll‐like receptor stimulation. These results provide a potential mechanism for the induction of responses to autoantigens unable to induce specific T‐cell responses, and provide new insights into polysaccharide‐specific immunity.</p> </abstract>