Enhanced shear‐induced platelet aggregation due to low‐temperature storage. Issue 7 (9th October 2012)

• Record Type: Journal Article
• Authors: Montgomery, Robbie K.; Reddoch, Kristin M.; Evani, Shankar J.; Cap, Andrew P.; Ramasubramanian, Anand K.
• Is Part Of: Transfusion. Volume 53:Issue 7(2013)
• Page Start: 1520
• Page End: 1530
• Publication Date: 2012-10-09
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/j.1537-2995.2012.03917.x
• Languages: English
• ISSNs: 0041-1132

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="trf3917-sec-0001" sec-type="section"> <title>Background</title> <p>Refrigeration of platelets (PLTs) offers an attractive alternative to the currently practiced storage at room temperature since it may mitigate problems associated with bacterial contamination and extend storage lifetime. Refrigeration causes a number of biophysical and biochemical changes in PLTs and decreases PLT circulation time in vivo. However, the effect of refrigeration on PLT hemostatic functions under physiologic and pathophysiologic shear conditions has not been adequately characterized.</p> </sec> <sec id="trf3917-sec-0002" sec-type="section"> <title>Study Design and Methods</title> <p>Washed PLTs prepared from either fresh PLT‐rich plasma (PRP) or PRP stored at 4°C for 2 days was mixed with exogenous von Willebrand factor (VWF) and fibrinogen and sheared in a cone‐and‐plate viscometer. PLT aggregation, activation, and VWF binding after shear and glycoprotein (GP) Ibα receptor expression and ristocetin‐induced PLT agglutination were measured.</p> </sec> <sec id="trf3917-sec-0003" sec-type="section"> <title>Results</title> <p>PLTs stored at 4°C for 2 days aggregated significantly more than fresh PLTs particularly at high shear rates (10, 000/sec), and this increase was independent of PLT concentration or suspension viscosity. Further, refrigerated PLTs showed a greater increase in GP Ibα–dependent PLT activation under shear and also bound more VWF than fresh PLTs. However, the GP Ibα expression levels as measured by three different antibodies were significantly lower in refrigerated PLTs than in fresh PLTs, and refrigeration resulted in a modest decrease in ristocetin‐induced PLT agglutination.</p> </sec> <sec id="trf3917-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The combined results demonstrate that refrigeration increases PLT aggregation under high shear, but not static, conditions and also increases shear‐induced VWF binding and PLT activation. Clinically, enhanced shear‐induced PLT aggregation due to low temperature storage may be a beneficial strategy to prevent severe bleeding in trauma.</p> </sec> </abstract>