Relationship between ABO genotype and A antigen expression on platelets. Issue 8 (12th November 2012)

• Record Type: Journal Article
• Authors: DeLelys, Michelle E.; Ochoa, Gorka; Cserti‐Gazdewich, Christine M.; Vietz, Christine; Preffer, Frederic I.; Dzik, Walter
• Is Part Of: Transfusion. Volume 53:Issue 8(2013)
• Page Start: 1763
• Page End: 1771
• Publication Date: 2012-11-12
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/j.1537-2995.2012.03952.x
• Languages: English
• ISSNs: 0041-1132

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="trf3952-sec-0001" sec-type="section"> <title>Background</title> <p>Although platelets (PLTs) are known to express ABH antigens, the extent of expression is different on PLTs compared with red blood cells and the relationship between PLT ABH expression and genotype has not been thoroughly investigated.</p> </sec> <sec id="trf3952-sec-0002" sec-type="section"> <title>Study Design and Methods</title> <p>We measured blood group H and A antigens on PLTs from 100 normal volunteers using fluorescent‐conjugated reagents and flow cytometry. Individuals were also genotyped at the <italic>ABO</italic> locus using a commercially available genotyping system.</p> </sec> <sec id="trf3952-sec-0003" sec-type="section"> <title>Results</title> <p>Expression of A and H antigen varied widely on PLTs from different individuals. Among group A and AB persons, H antigen expression was significantly greater than A antigen (p < 0.0001). The ratio of H‐to‐A antigen expression varied more than 100‐fold in a predictable fashion according to genotype with values lowest in <italic>A<sup>1</sup>/A<sup>1</sup></italic> < <italic>A<sup>1</sup>/O</italic> < <italic>A<sup>2</sup>/A<sup>2</sup></italic> < <italic>A<sup>2</sup>/O</italic>. H and A antigen expression was unaffected by secretor status. The proportion of PLTs with high A expression also varied directly according to genotype.</p> </sec> <sec id="trf3952-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Blood group A and H antigen expression on PLTs varies in a predictable fashion according to genotype. Flow cytometry and genotyping identify individuals who strongly express A antigens, a finding that may be relevant to clinical PLT transfusion across ABO barriers.</p> </sec> </abstract>