RHCE*ceTI encodes partial c and partial e and is often in cis to RHD*DIVa. Issue 4 (13th July 2012)

• Record Type: Journal Article
• Authors: Westhoff, Connie M.; Vege, Sunitha; Halter Hipsky, Christine; Hue‐Roye, Kim; Copeland, Tamara; Velliquette, Randall W.; Horn, Trina; Lomas‐Francis, Christine; Reid, Marion E.
• Is Part Of: Transfusion. Volume 53:Issue 4(2013)
• Page Start: 741
• Page End: 746
• Publication Date: 2012-07-13
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/j.1537-2995.2012.03800.x
• Languages: English
• ISSNs: 0041-1132

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>BACKGROUND:</bold> In the Rh blood group system, variant RhD and RhCE express several partial antigens. We investigated <italic>RH</italic> in samples with partial DIVa that demonstrated weak and variable reactivity with anti‐C.</p> <p> <bold>STUDY DESIGN AND METHODS:</bold> Standard hemagglutination techniques, polymerase chain reaction–based assays, and <italic>RH</italic> sequencing were used.</p> <p> <bold>RESULTS:</bold> DNA analysis showed that six red blood cell (RBC) samples with weak and inconsistent reactivity with anti‐C lacked <italic>RHCE</italic>*<italic>C</italic>, but all had <italic>RHD</italic>*<italic>DIVa</italic>, which encodes partial D and Go<sup>a</sup>. We then tested RBCs from 19 Go(a+) cryopreserved samples (confirmed to have <italic>RHD</italic>*<italic>DIVa</italic>) with four anti‐C and observed weak variable reactions. <italic>RHCE</italic> genotyping found all but one of the samples with <italic>RHD</italic>*<italic>DIVa</italic> also had <italic>RHCE</italic> nt 48G>C and 1025C>T, named <italic>RHCE</italic>*<italic>ceTI</italic>. Lookback of samples referred for workup and found to have either allele revealed 47 of 55 had both <italic>RHD</italic>*<italic>DIVa</italic> and <italic>RHCE</italic>*<italic>ceTI</italic>, four had <italic>RHD</italic>*<italic>DIVa</italic> without <italic>RHCE</italic>*<italic>ceTI</italic>, and four had <italic>RHCE</italic>*<italic>ceTI</italic> without <italic>RHD</italic>*<italic>DIVa</italic>. Alloanti‐c was found in a patient with c+ RBCs and <italic>RHCE</italic>*<italic>ceTI</italic> in trans to <italic>RHCE</italic>*<italic>Ce</italic>, and alloanti‐e was found in a patient with e+ RBC and <italic>RHCE</italic>*<italic>ceTI</italic> in trans to <italic>RHCE</italic>*<italic>cE</italic>. <italic>RHD</italic>*<italic>DIVa</italic> in trans to <italic>RHD</italic> erroneously tested as <italic>RHD</italic> hemizygous.</p> <p> <bold>CONCLUSIONS:</bold> <italic>RHD</italic>*<italic>DIVa</italic> and <italic>RHCE</italic>*<italic>ceTI</italic> almost always, but not invariably, travel together. This haplotype is found in people of African ancestry and the RBCs can demonstrate aberrant reactivity with anti‐C. <italic>RHCE</italic>*<italic>ceTI</italic> encodes partial c and e antigens. We confirm that <italic>RHD</italic> zygosity assays are unreliable in samples with <italic>RHD</italic>*<italic>DIVa</italic>.</p> </abstract>