Back Cover: Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering (Chem. Eur. J. 22/2013). Issue 22 (17th May 2013)

• Record Type: Journal Article
• Authors: Wilding, Birgit; Winkler, Margit; Petschacher, Barbara; Kratzer, Regina; Egger, Sigrid; Steinkellner, Georg; Lyskowski, Andrzej; Nidetzky, Bernd; Gruber, Karl; Klempier, Norbert
• Is Part Of: Chemistry. Volume 19:Issue 22(2013)
• Page Start: 7252
• Page End: 7252
• Publication Date: 2013-05-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201390080
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Nitrile reductase QueF</bold> catalyzes the reduction of a nitrile to its corresponding amine. In their Full Paper on <ext-link ext-link-type="doi" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">page 7007 ff.</ext-link>, N. Klempier et al. report on the investigation of active‐site binding and the substrate scope of <italic>E. coli</italic> QueF by modeling, enzyme, and substrate engineering. The active‐site residues and structural features of the substrates essential for catalysis were identified in spectrophotometric and HPLC‐MS based screenings of active‐site mutants and natural substrate analogues.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg20p7n99w" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>