Real‐Time DNP1 NMR Observations of Acetic Acid Uptake, Intracellular Acidification, and of Consequences for Glycolysis and Alcoholic Fermentation in Yeast. Issue 40 (9th September 2013)

• Record Type: Journal Article
• Authors: Jensen, Pernille R.; Karlsson, Magnus; Lerche, Mathilde H.; Meier, Sebastian
• Is Part Of: Chemistry. Volume 19:Issue 40(2013)
• Page Start: 13288
• Page End: 13293
• Publication Date: 2013-09-09
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201302429
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Uptake and upshot in vivo</bold>: Straightforward methods that permit the real‐time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular‐reaction networks are crucial for improved bioprocess monitoring and control (see scheme). Herein, dynamic nuclear polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg3hfpj9zw" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>