Selective Chemical Labelling of 5‐Formylcytosine in DNA by Fluorescent Dyes. Issue 19 (19th March 2013)

• Record Type: Journal Article
• Authors: Hu, Jianlin; Xing, Xiwen; Xu, Xiaowei; Wu, Fan; Guo, Pu; Yan, Shengyong; Xu, Zhenghao; Xu, Jiahui; Weng, Xiaocheng; Zhou, Xiang
• Is Part Of: Chemistry. Volume 19:Issue 19(2013)
• Page Start: 5836
• Page End: 5840
• Publication Date: 2013-03-19
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201300082
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Direct labelling</bold>: 5‐Formylcytosine in DNA can be selectively labelled by fluorescent dyes containing an active amino group. The labelled DNA shows strong fluorescence and can be detected by polyacrylamide gel electrophoresis (PAGE) and fluorescence measurements (see scheme). This method can distinguish 5‐formylcytosine from other methylation forms of cytosine in DNA.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1ztb0q4f" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>