A Peptide Nucleic Acid (PNA) Heteroduplex Probe Containing an Inosine–Cytosine Base Pair Discriminates a Single‐Nucleotide Difference in RNA. Issue 16 (13th March 2013)

• Record Type: Journal Article
• Authors: Matsumoto, Katsuhiko; Nakata, Eiji; Tamura, Tomoki; Saito, Isao; Aizawa, Yasunori; Morii, Takashi
• Is Part Of: Chemistry. Volume 19:Issue 16(2013)
• Page Start: 5034
• Page End: 5040
• Publication Date: 2013-03-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201204183
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Selective discrimination of a single‐nucleotide difference in single‐stranded DNA or RNA remains a challenge with conventional DNA or RNA probes. A peptide nucleic acid (PNA)‐derived probe, in which PNA forms a pseudocomplementary heteroduplex with inosine‐containing DNA or RNA, effectively discriminates a single‐nucleotide difference in a closely related group of sequences of single‐stranded DNA and/or RNA. The pseudocomplementary PNA heteroduplex is easily converted to a fluorescent probe that distinctively detects a member of highly homologous let‐7 microRNAs.</p> </abstract>