A Novel High‐Speed Production Process to Create Modular Components for the Bottom‐Up Assembly of Large‐Scale Tissue‐Engineered Constructs. Issue 1 (3rd June 2014)

• Record Type: Journal Article
• Authors: Khan, Omar F.; Voice, Derek N.; Leung, Brendan M.; Sefton, Michael V.
• Is Part Of: Advanced healthcare materials. Volume 4:Issue 1(2015)
• Page Start: 113
• Page End: 120
• Publication Date: 2014-06-03
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201400150
• Languages: English
• ISSNs: 2192-2640

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>To replace damaged or diseased tissues, large tissue‐engineered constructs can be prepared by assembling modular components in a bottom‐up approach. However, a high‐speed method is needed to produce sufficient numbers of these modules for full‐sized tissue substitutes. To this end, a novel production technique is devised, combining air shearing and a plug flow reactor‐style design to rapidly produce large quantities of hydrogel‐based (here type I collagen) cylindrical modular components with tunable diameters and length. Using this technique, modules containing NIH 3T3 cells show greater than 95% viability while endothelial cell surface attachment and confluent monolayer formation are demonstrated. Additionally, the rapidly produced modules are used to assemble large tissue constructs (&gt;1 cm³) in vitro. Module building blocks containing luciferase‐expressing L929 cells are packed in full size adult rat‐liver‐shaped bioreactors and perfused with cell medium, to demonstrate the capacity to build organ‐shaped constructs; bioluminescence demonstrates sustained viability over 3 d. Cardiomyocyte‐embedded modules are also used to assemble electrically stimulatable contractile tissue.</p> </abstract>