Ncor1 and Ncor2 play essential but distinct roles in zebrafish primitive myelopoiesis. Issue 12 (11th September 2014)

• Record Type: Journal Article
• Authors: Li, Jingyun; Li, Kui; Dong, Xiaohua; Liang, Dong; Zhao, Qingshun
• Is Part Of: Developmental dynamics. Volume 243:Issue 12(2014:Dec.)
• Page Start: 1544
• Page End: 1553
• Publication Date: 2014-09-11
• Subjects: Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.24181
• Languages: English
• ISSNs: 1058-8388

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <underline> <bold>Background:</bold> </underline> Although Ncor1 and Ncor2, the co‐repressors that can actively repress gene transcription through binding nuclear receptors in the absence of ligands, are crucial to vertebrate embryogenesis, their roles in its primitive myelopoiesis remain unknown. We investigated the function of <italic>ncor1</italic> or <italic>ncor2</italic> in zebrafish embryos by antisense morpholino knocking down technologies. <underline><bold>Results:</bold></underline> Development of both <italic>mfap4</italic><sup>+</sup> macrophages and <italic>mpx</italic><sup>+</sup> neutrophils was abolished in <italic>ncor2</italic> morphants, whereas development of <italic>mpx</italic><sup>+</sup> neutrophils was depleted in <italic>ncor1</italic> morphants. <italic>ncor2</italic> was essential to the development of <italic>spi1b<sup>+</sup></italic> myeloid precursors but not anterior hemangioblasts whereas <italic>ncor1</italic> was dispensable to the specification of <italic>spi1b<sup>+</sup></italic> myeloid precursors and anterior hemangioblasts. Overexpressing <italic>spi1b</italic> could partially rescue expressions of <italic>mfap4</italic> and <italic>mpx</italic> in <italic>ncor2</italic> morphants. Furthermore, overexpressing <italic>tal1/lmo2</italic> could well rescue the defective myelopoiesis in both <italic>ncor1</italic> and <italic>ncor2</italic> morphants. <underline><bold>Conclusions:</bold></underline> Ncor1 and Ncor2 play essential but distinct roles in zebrafish primitive myelopoiesis. <italic>ncor2</italic> could parallel with <italic>tal1/lmo2</italic> and acted upstream of <italic>spi1b</italic> to produce mature macrophages and neutrophils during primitive myelopoiesis. The role of <italic>ncor1</italic> in zebrafish myelopoiesis could be substituted by excessive Tal1/Lmo2. <italic>Developmental Dynamics 243:1544–1553, 2014</italic>. © 2014 Wiley Periodicals, Inc.</p> </abstract>