Environment‐Sensitive Probes Containing a 2, 6‐Diethynylpyridine Motif for Fluorescence Turn‐On Detection and Induction of Nanoarchitectures of Human Telomeric Quadruplex. Issue 50 (21st October 2014)

• Record Type: Journal Article
• Authors: Das, Rabindra Nath; Debnath, Manish; Gaurav, Abhiket; Dash, Jyotirmayee
• Is Part Of: Chemistry. Volume 20:Issue 50(2014)
• Page Start: 16688
• Page End: 16693
• Publication Date: 2014-10-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201404795
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Bis(phenylethynyl)pyridylcarboxamides with amide side chains at the <italic>para</italic> position of the NH₂ group possess strong solvatochromic properties compared with the <italic>meta</italic> analogues. Fluorescence binding titrations show that these probes exhibit remarkable fluorescence turn‐on responses upon interacting with the human telomeric G‐quadruplex (<italic>h‐TELO</italic>). Förster resonance energy transfer melting analysis shows the high selectivity of these probes for <italic>h‐TELO</italic> over duplex DNA. Isothermal titration calorimetry, as well as UV/Vis and fluorescence spectroscopy studies, show that the <italic>meta</italic> analogue has a twofold binding affinity for <italic>h‐TELO</italic> over the <italic>para</italic> analogue. The noncovalent interaction of these small‐molecule probes with <italic>h‐TELO</italic> has been used to regulate the assembly of novel supramolecular nanoarchitectures.</p> </abstract>