Involvement of Ralfuranone Production in the Virulence of Ralstonia solanacearum OE1‐1. Issue 17 (22nd September 2014)

• Record Type: Journal Article
• Authors: Kai, Kenji; Ohnishi, Hideyuki; Mori, Yuka; Kiba, Akinori; Ohnishi, Kouhei; Hikichi, Yasufumi
• Is Part Of: Chembiochem. Volume 15:Issue 17(2014)
• Page Start: 2590
• Page End: 2597
• Publication Date: 2014-09-22
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201402404
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p> <italic>Ralstonia solanacearum</italic> causes a destructive disease called "bacterial wilt" in numerous plant species. Its virulence is controlled by the transcriptional regulator PhcA, the activity of which is, in turn, regulated in a cell‐density dependent manner, termed quorum sensing. We herein described the identification and characterization of ralfuranones J–L, new PhcA‐regulated secondary metabolites, and the known derivatives, ralfuranones A and B, from <italic>R. solanacearum</italic> strain OE1‐1. Their structures were determined by spectroscopic and chemical methods. These ralfuranones were also detected in vascular exudates from host plants infected with OE1‐1. Deletion of <italic>ralA</italic>, which encodes an enzyme for ralfuranone biosynthesis, reduced the virulence of OE1‐1 in tomato plants. Virulence was restored by complementation of the <italic>ralA</italic> gene. The results suggest that ralfuranones play important roles in the virulence of OE1‐1.</p> </abstract>