Liquid chromatography–tandem mass spectrometric assay for the determination of vaccarin in rat plasma: application to a pharmacokinetic study. (7th May 2014)

• Record Type: Journal Article
• Authors: Yang, Guilin; Zhang, Nan; Wang, Tiance; Zhang, Shudong; Xu, Rihao; Zhu, Zhicheng; Liu, Kexiang
• Is Part Of: Biomedical chromatography. Volume 28:Number 12(2014:Dec.)
• Page Start: 1789
• Page End: 1794
• Publication Date: 2014-05-07
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3222
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Vaccarin, a flavonoid glycoside, is considered one of the major active constituents of <italic>Vaccaria segetalis</italic>. A simple and specific liquid chromatography–tandem mass spectrometric method was developed and validated for quantifying vaccarin in rat plasma following intravenous dosing. Plasma samples were precipitated with methanol and separated on a Venusil‐C₁₈ analytical column (2.1 × 50 mm, 5 µm particles) with gradient elution consisting of methanol and 0.1% (v/v) formic acid as the mobile phase. The detection was performed on an Agilent Triple Quad LC/MS with electrospray ionization inlet in the positive multiple reaction monitoring mode. Good linearity was achieved over the concentration range of 12.5–25, 000 ng/mL (<italic>r</italic>² &gt; 0.99). Intra‐ and inter‐day precisions were &lt;9.1%, and accuracy ranged from −2.8 to 8.7%. The lower limit of quantification for vaccarin was 12.5 ng/mL, and the analyte was stable under various storage conditions. This validated method was successfully applied to the preliminary pharmacokinetic studies of vaccarin following intravenous administrations of 1.21, 2.41, and 4.82 mg/kg vaccarin in rats. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>