Reversible Ca2+ Switch of An Engineered Allosteric Antioxidant Selenoenzyme1. Issue 49 (6th October 2014)

• Record Type: Journal Article
• Authors: Zhang, Chunqiu; Pan, Tiezheng; Salesse, Christian; Zhang, Dongmei; Miao, Lu; Wang, Liang; Gao, Yuzhou; Xu, Jiayun; Dong, Zeyuan; Luo, Quan; Liu, Junqiu
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 49(2014)
• Page Start: 13536
• Page End: 13539
• Publication Date: 2014-10-06
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201407135
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A Ca²⁺‐responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center of glutathione peroxidase (GPx). By combining the recognition capacity for the glutathione (GSH) substrate and the steric orientation of the catalytic selenium moiety, the engineered selenium‐containing recoverin exhibits high GPx activity for the catalyzed reduction of H₂O₂ by glutathione (GSH). Moreover, the engineered selenoenzyme can be switched on/off by Ca²⁺‐induced allosterism of the protein recoverin. This artificial selenoenzyme also displays excellent antioxidant ability when it was evaluated using a mitochondrial oxidative damage model, showing great potential for controlled catalysis in biomedical applications.</p> </abstract>