In Vivo Residue‐Specific Dopa‐Incorporated Engineered Mussel Bioglue with Enhanced Adhesion and Water Resistance1. Issue 49 (4th September 2014)

• Record Type: Journal Article
• Authors: Yang, Byeongseon; Ayyadurai, Niraikulam; Yun, Hyungdon; Choi, Yoo Seong; Hwang, Byeong Hee; Huang, Jun; Lu, Qingye; Zeng, Hongbo; Cha, Hyung Joon
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 49(2014)
• Page Start: 13360
• Page End: 13364
• Publication Date: 2014-09-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201406099
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Misaminoacylation of 3, 4‐dihydroxyphenylalanine (Dopa) molecules to tRNA^Tyr by endogenous tyrosyl‐tRNA synthetase allowed the quantitative replacement of tyrosine residues with a yield of over 90 % by an in vivo residue‐specific incorporation strategy, to create, for the first time, engineered mussel adhesive proteins (MAPs) in <italic>Escherichia coli</italic> with a very high Dopa content, close to that of natural MAPs. The Dopa‐incorporated MAPs exhibited a superior surface adhesion and water resistance ability by assistance of Dopa‐mediated interactions including the oxidative Dopa cross‐linking, and furthermore, showed underwater adhesive properties comparable to those of natural MAPs. These results propose promising use of Dopa‐incorporated engineered MAPs as bioglues or adhesive hydrogels for practical underwater applications.</p> </abstract>