Protective effects of N-acetylcysteine on triamcinolone acetonide-induced lens damage in rats. (December 2014)

• Record Type: Journal Article
• Authors: Tuzcu, Esra Ayhan; Tuzcu, Kasim; Basarslan, Fatmagul; Motor, Sedat; Coskun, Mesut; Keskin, Ugurcan; Ayintap, Emre; Ilhan, Ozgur; Oksuz, Huseyin
• Is Part Of: Cutaneous and ocular toxicology. Volume 33:Number 4(2014:Dec.)
• Page Start: 294
• Page End: 298
• Publication Date: 2014-12
• Subjects: Dermatotoxicology -- Periodicals; Ocular toxicology -- Periodicals; Ocular pharmacology -- Periodicals; Dermatopharmacology -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.3109/15569527.2013.857679
• Languages: English
• ISSNs: 1556-9527

Abstract

<abstract> <title>Abstract</title> <p> <italic>Objective</italic>: To examine the relationship of cataract forming effect of intravitreal triamcinolone acetonide (IVTA) injection with oxidative status and the effect of N-acetylcysteine (NAC) on these alterations.</p> <p> <italic>Materials and methods</italic>: Twenty-six Wistar-Albino rats were included in the study. Rats were assigned into four groups as follows: intravitreal saline injection group (controls); IVTA injection group; IVTA + intraperitoneal NAC injection group (IVTA + NAC); and intraperitoneal NAC injection group (NAC). Triamcinolone acetonide was intravitreally injected at a dose of 1 mg. NAC was intraperitoneally injected at a dose of 150 µg/g body weight. Animals were sacrificed and lens specimens were analyzed for levels of malondialdehyde (MDA) and protein carbonyl (PC) and activities of glutathione (GSH) and glutathione peroxidase (GSH-Px).</p> <p> <italic>Results</italic>: We found that the MDA and PC levels of lenses were increased in the IVTA group (<italic>p</italic> &lt; 0.01). It was seen that GSH and GSH-Px in lenses were decreased in the IVTA group (<italic>p</italic> &lt; 0.01). NAC administration significantly ameliorated these changes in the IVTA + NAC group (<italic>p</italic> &lt; 0.05).</p> <p> <italic>Conclusion</italic>: These results indicate that the NAC produces a protective mechanism against IVTA-induced cataract and suggest a role of oxidative stress in pathogenesis.</p> </abstract>