Morphological and molecular phylogenetic analysis of the high triglyceride‐producing marine diatom, Fistulifera solaris sp. nov. (Bacillariophyceae). Issue 4 (17th September 2014)

• Record Type: Journal Article
• Authors: Matsumoto, Mitsufumi; Mayama, Shigeki; Nemoto, Michiko; Fukuda, Yorikane; Muto, Masaki; Yoshino, Tomoko; Matsunaga, Tadashi; Tanaka, Tsuyoshi
• Is Part Of: Phycological research. Volume 62:Issue 4(2014:Oct.)
• Page Start: 257
• Page End: 268
• Publication Date: 2014-09-17
• Subjects: Algae -- Japan -- Periodicals; Algae -- Periodicals; 579.8
• DOI: 10.1111/pre.12066
• Languages: English
• ISSNs: 1322-0829

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p> <italic>Fistulifera</italic> sp. strain JPCC DA0580, a marine pennate diatom, contains extremely high levels of intracellular triglyceride and has been suggested as a promising source of feedstock for biodiesel fuels. JPCC DA0580 was isolated from a mangrove swamp located in Sumiyo Bay below the mouths of the Sumiyo and Yakugachi Rivers in Amami‐Ohshima, Kagoshima, Japan. In this study, the morphology and the 18S rDNA sequence of JPCC DA0580 were compared with those of other <italic>Fistulifera</italic> strains. JPCC DA0580 possesses morphological characters of the genus <italic>Fistulifera</italic>, namely lightly silicified frustules, a distinct median costa (raphe sternum), and a wart‐like central pore (fistula). Morphometric analysis revealed that JPCC DA0580 differs from other <italic>Fistulifera</italic> species by the presence of a valve with coarser striation and coarser areolation. On the basis of 18S rDNA phylogeny, JPCC DA0580 formed a well‐supported clade with other members of the <italic>Fistulifera</italic> species complex, although the number of nucleotide substitutions was highest in JPCC DA0580. Our results led us to propose the taxonomic name <italic>Fistulifera solaris</italic> sp. nov. for JPCC DA0580.</p> </abstract>