Evaluation of BSP expression and apoptosis in the periodontal ligament during orthodontic relapse: a preliminary study. (13th June 2014)

• Record Type: Journal Article
• Authors: McManus, A.; Utreja, A.; Chen, J.; Kalajzic, Z.; Yang, W.; Nanda, R.; Wadhwa, S.; Uribe, F.
• Is Part Of: Orthodontics and craniofacial research. Volume 17:Number 4(2014:Nov.)
• Page Start: 239
• Page End: 248
• Publication Date: 2014-06-13
• Subjects: Skull -- Growth -- Periodicals; Facial bones -- Growth -- Periodicals; Orthodontics -- Periodicals; Orthodontics, Corrective -- Periodicals; Orthodontic appliances -- Periodicals; 617.51
• DOI: 10.1111/ocr.12049
• Languages: English
• ISSNs: 1601-6335

Abstract

<abstract abstract-type="main" id="ocr12049-abs-0001"> <title>Structured Abstract</title> <sec id="ocr12049-sec-0001" sec-type="section"> <title>Objective</title> <p>To examine the expression of bone sialoprotein (BSP) and apoptosis in an <italic>in vivo</italic> orthodontic relapse model.</p> </sec> <sec id="ocr12049-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Male mice (10–12 weeks old), either transgenic [green fluorescent protein (GFP) driven by the BSP promoter] or wild type, were used in this study. To achieve orthodontic tooth movement (OTM), maxillary right first molars were moved mesially using closed‐coil springs. Animals were divided into an OTM group (14 days continuous orthodontic force – 11 animals) or Relapse group (10 days of force application followed by 4 days of relapse – 8 animals). The control group was comprised of the contralateral maxillary molars. The periodontal ligament (PDL) was analyzed in areas of compression and tension for transgenic expression, osteoclast localization, and the presence of apoptotic cells.</p> </sec> <sec id="ocr12049-sec-0003" sec-type="section"> <title>Results</title> <p>There was a significant decrease in GFP‐labeled cells on the compression and tension sides of the PDL in the OTM group compared with control. In the relapse group, GFP‐labeled cells were significantly decreased only on the old compression side. Osteoclasts were localized on the compression side of the OTM group, whereas in the Relapse group, they were present on both sides. PDL apoptosis significantly increased on the compression side in OTM and Relapse groups.</p> </sec> <sec id="ocr12049-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Both OTM and Relapse groups exhibited a decreased number of GFP‐labeled cells in areas of compression and tension. There was significant PDL apoptosis in regions under compressive forces following OTM and to a lesser extent following relapse.</p> </sec> </abstract>