Microbial signature profiles of periodontally healthy and diseased patients. (22nd September 2014)

• Record Type: Journal Article
• Authors: Lourenço, Talita Gomes Baêta; Heller, Débora; Silva‐Boghossian, Carina Maciel; Cotton, Sean L.; Paster, Bruce J.; Colombo, Ana Paula Vieira
• Is Part Of: Journal of clinical periodontology. Volume 41:Number 11(2014:Nov.)
• Page Start: 1027
• Page End: 1036
• Publication Date: 2014-09-22
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12302
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12302-abs-0001"> <title>Abstract</title> <sec id="jcpe12302-sec-0001" sec-type="section"> <title>Aim</title> <p>To determine microbial profiles that discriminate periodontal health from different forms of periodontal diseases.</p> </sec> <sec id="jcpe12302-sec-0002" sec-type="section"> <title>Methods</title> <p>Subgingival biofilm was obtained from patients with periodontal health (27), gingivitis (11), chronic periodontitis (35) and aggressive periodontitis (24), and analysed for the presence of >250 species/phylotypes using HOMIM. Microbial differences among groups were examined by Mann–Whitney <italic>U</italic>‐test. Regression analyses were performed to determine microbial risk indicators of disease.</p> </sec> <sec id="jcpe12302-sec-0003" sec-type="section"> <title>Results</title> <p>Putative and potential new periodontal pathogens were more prevalent in subjects with periodontal diseases than periodontal health. Detection of <italic>Porphyromonas endodontalis/Porphyromonas</italic> spp. (OR 9.5 [1.2–73.1]) and <italic>Tannerella forsythia</italic> (OR 38.2 [3.2–450.6]), and absence of <italic>Neisseria polysaccharea</italic> (OR 0.004 [0–0.15]) and <italic>Prevotella denticola</italic> (OR 0.014 [0–0.49], <italic>p</italic> < 0.05) were risk indicators of periodontal disease. Presence of <italic>Aggregatibacter actinomycetemcomitans</italic> (OR 29.4 [3.4–176.5]), <italic>Cardiobacterium hominis</italic> (OR 14.9 [2.3–98.7]), <italic>Peptostreptococcaceae</italic> sp. (OR 35.9 [2.7–483.9]<italic>), P. alactolyticus</italic> (OR 31.3 [2.1–477.2]), and absence of <italic>Fretibacterium</italic> spp. (OR 0.024 [0.002–0.357]), <italic>Fusobacterium naviforme/Fusobacterium nucleatum ss vincentii</italic> (OR 0.015 [0.001–0.223]), <italic>Granulicatella adiacens/Granulicatella elegans</italic> (OR 0.013 [0.001–0.233], <italic>p</italic> < 0.05) were associated with aggressive periodontitis.</p> </sec> <sec id="jcpe12302-sec-0004" sec-type="section"> <title>Conclusion</title> <p>There were specific microbial signatures of the subgingival biofilm that were able to distinguish between microbiomes of periodontal health and diseases. Such profiles may be used to establish risk of disease.</p> </sec> </abstract>