Roles of Edc3 in the oxidative stress response and CaMCA1‐encoded metacaspase expression in Candida albicans. (19th September 2014)

• Record Type: Journal Article
• Authors: Jung, Jong‐Hwan; Kim, Jinmi
• Is Part Of: FEBS journal. Volume 281:Number 21(2014)
• Page Start: 4841
• Page End: 4851
• Publication Date: 2014-09-19
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.13022
• Languages: English
• ISSNs: 1742-464X

Abstract

<abstract abstract-type="main" id="febs13022-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The Edc3 protein is an enhancer of mRNA decapping, and acts as a scaffold protein for the mRNA granules that are known as processing bodies in yeast. In the pathogenic yeast <italic>Candida albicans</italic>, various stresses, such as glucose depletion, oxidative stress, and filamentation defects, induce the accumulation of processing bodies. Here, we report that the <italic>edc3</italic>/<italic>edc3</italic> deletion strain showed increased resistance to various stresses, including hydrogen peroxide, acetic acid, and high temperature. Oxidative stress is known to induce the intracellular accumulation of reactive oxygen species (ROS) and apoptotic cell death in <italic>C. albicans</italic>. We found that the ROS level was lower in <italic>edc3/edc3</italic> cells than in wild‐type cells following oxidative stress. We also observed that expression of the metacaspase gene <italic>CaMCA1</italic> was decreased in <italic>edc3</italic>/<italic>edc3</italic> cells. Overexpression of <italic>CaMCA1</italic> suppressed the decreased accumulation of ROS and the increased resistance to hydrogen peroxide in <italic>edc3</italic>/<italic>edc3</italic> cells. The catalase Cat1 and the superoxide dismutase Sod1 were upregulated in <italic>edc3</italic>/<italic>edc3</italic> cells as compared with wild‐type cells. On the basis of these findings, we suggest that <italic>EDC3</italic> plays a critical role in the expression of <italic>CaMCA1</italic> and the oxidative stress response in <italic>C. albicans</italic>.</p> </abstract>