Cord blood CD34+ cells expanded on Wharton's jelly multipotent mesenchymal stromal cells improve the hematopoietic engraftment in NOD/SCID mice. (26th May 2014)

• Record Type: Journal Article
• Authors: Milazzo, Luisa; Vulcano, Francesca; Barca, Alessandra; Macioce, Giampiero; Paldino, Emanuela; Rossi, Stefania; Ciccarelli, Carmela; Hassan, Hamisa J.; Giampaolo, Adele
• Is Part Of: European journal of haematology. Volume 93:Number 5(2014:Nov.)
• Page Start: 384
• Page End: 391
• Publication Date: 2014-05-26
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Blood -- Periodicals; 616.15005
• DOI: 10.1111/ejh.12363
• Languages: English
• ISSNs: 0902-4441

Abstract

<abstract abstract-type="main" id="ejh12363-abs-0001"> <title>Abstract</title> <sec id="ejh12363-sec-0001" sec-type="section"> <title>Objective</title> <p>This study aims to investigate the capability of Wharton's jelly multipotent mesenchymal stromal cells (WJ‐MSC) to support the <italic>in vitro</italic> expansion of hematopoietic stem/progenitor cells (HSPC) derived from cord blood (CB) in the absence of exogenous cytokines, and the effect on engraftment of the expanded cells in a mouse model.</p> </sec> <sec id="ejh12363-sec-0002" sec-type="section"> <title>Methods</title> <p>CB‐CD34+ cells were seeded on WJ‐MSC layer and cultured in HP01 serum‐free medium. Day‐7 and day‐13 expanded cells were transplanted in NOD/SCID mice. After 8 wk, engraftment was evaluated in mouse bone marrow as percentage of human CD45+ cells.</p> </sec> <sec id="ejh12363-sec-0003" sec-type="section"> <title>Results</title> <p>CD34+ population was expanded without increasing the differentiation rate. Co‐culture increased the expansion of the CD34+ cells by 2.0 and 7.3 times after 7 and 13 d, respectively, and maintained the CD34+ cells up to day 20. In particular, earlier CD34+/CD90+ and CD34+/CD33− subtypes were increased. An advantage of the day‐7 co‐cultured HSPC in respect of HSPC at day 0 in the engraftment of NOD/SCID mice was obtained both as percentage of mice engrafted (100% vs. 75%) and as percentage of chimerism.</p> </sec> <sec id="ejh12363-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Although the increase in hematopoietic progenitors is not dramatic as in the presence of added cytokines, this study demonstrates the effectiveness of the WJ‐MSC not only to preserve the CD34+ population but also to improve the repopulating efficacy of the amplified HSPC, also in the absence of added cytokines and growth factors.</p> </sec> </abstract>