Melatonin induces apoptosis through biomolecular changes, in SK‐LU‐1 human lung adenocarcinoma cells. (27th October 2014)

• Record Type: Journal Article
• Authors: Plaimee, P.; Weerapreeyakul, N.; Thumanu, K.; Tanthanuch, W.; Barusrux, S.
• Is Part Of: Cell proliferation. Volume 47:Number 6(2014:Dec.)
• Page Start: 564
• Page End: 577
• Publication Date: 2014-10-27
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12140
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12140-abs-0001"> <title>Abstract</title> <sec id="cpr12140-sec-0001" sec-type="section"> <title>Objectives</title> <p>Anti‐cancer effects of melatonin (<italic>N</italic>‐acetyl‐5‐methoxytryptamine, an indole‐amine), have been widely reported, however, little has been known, regarding its mechanism(s) of action in lung cancer. Thus, we investigated its induction of apoptosis through biomolecular changes (lipid, protein and nucleic acid/DNA) in the SK‐LU‐1 human lung cancer cell line.</p> </sec> <sec id="cpr12140-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>We used Fourier transform infrared (FTIR) microspectroscopy, and conventional methods, to confirm changes in lipid (annexin V/PI staining for membrane alteration), protein (caspase‐3/7 protein activity) and DNA (DAPI staining for DNA fragmentation).</p> </sec> <sec id="cpr12140-sec-0003" sec-type="section"> <title>Results</title> <p>We observed from FTIR data that melatonin increased lipid content and reduced intensity of nucleic acid/DNA, confirmed by annexin V/PI and DAPI respectively. Secondary protein structure at 1656 cm<sup>−1</sup> (α‐helix) was reduced and peak position of β‐sheet structure (1637 cm<sup>−1</sup>) was shifted to lower frequency. Alteration in apoptotic proteins was demonstrated <italic>via</italic> caspase‐3/7 activity induction.</p> </sec> <sec id="cpr12140-sec-0004" sec-type="section"> <title>Conclusions</title> <p>High melatonin concentration exerted anti‐cancer effects by changing biomolecular structure of lipids, nucleic acids and proteins, supporting its enhancement of apoptotic induction.</p> </sec> </abstract>