Occurrence of 'Candidatus Phytoplasma ziziphi' in apple trees in China. Issue 5 (5th June 2014)

• Record Type: Journal Article
• Authors: Li, Z.‐N.; Bai, Y.‐B.; Liu, P.; Zhang, L.; Wu, Y.‐F.; Balci, Y.
• Is Part Of: Forest pathology. Volume 44:Issue 5(2014:Oct.)
• Page Start: 417
• Page End: 419
• Publication Date: 2014-06-05
• Subjects: Trees -- Diseases and pests -- Periodicals; Trees -- Effect of air pollution on -- Periodicals; Forests and forestry -- Environmental aspects -- Periodicals; 634.9
• DOI: 10.1111/efp.12126
• Languages: English
• ISSNs: 1437-4781

Abstract

<abstract abstract-type="main" id="efp12126-abs-0001"> <title>Summary</title> <p>Young apple trees exhibiting symptoms of little leaf, margin involute and yellows were observed in an open‐air nursery in Yangling, Shaanxi, China. Transmission electron microscopy showed typical phytoplasma bodies in the sieve tube elements of symptomatic leaf samples. In two nested polymerase chain reaction (PCR) assays, products of expected size of 1.1 and 1.2 kb were separately generated from the total DNAs of symptomatic samples. A restriction fragment length polymorphism (RFLP) analysis of the purified 1.2 kb PCR products indicated that the disease associated with apple trees was '<italic>Ca</italic>. Phytoplasma ziziphi'. To our knowledge, this is the first report of '<italic>Ca</italic>. Phytoplasma ziziphi' infecting apple trees in China.</p> </abstract>