The bone marrow stromal compartment in multiple myeloma patients retains capability for osteogenic differentiation in vitro: defining the stromal defect in myeloma. (31st July 2014)

• Record Type: Journal Article
• Authors: Kassen, Deepika; Moore, Sally; Percy, Laura; Herledan, Gaelle; Bounds, Danton; Rodriguez‐Justo, Manuel; Croucher, Peter; Yong, Kwee
• Is Part Of: British journal of haematology. Volume 167:Number 2(2014:Oct.)
• Page Start: 194
• Page End: 206
• Publication Date: 2014-07-31
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; 616.15
• DOI: 10.1111/bjh.13020
• Languages: English
• ISSNs: 0007-1048

Abstract

<abstract abstract-type="main" id="bjh13020-abs-0001"> <title>Summary</title> <p>Defects in bone repair contribute to multiple myeloma (MM) bone disease. It is unknown whether this reflects failure of osteogenic differentiation from mesenchymal stromal cells (MSC), inherent stromal defects or mature cell dysfunction. We quantified the number of fibroblast colony‐forming units (CFU‐f) and osteoblast colony‐forming units (CFU‐ob) in freshly isolated bone marrow (BM) from healthy individuals (<italic>N</italic> = 10) and MM patients (<italic>N</italic> = 54). CFU‐f and CFU‐ob were present in MM BM, at comparable frequency to normal subjects, irrespective of disease stage, and the presence of bone disease. Adherent cultures from MM BM are able to differentiate into osteoblasts, as indicated by the early upregulation of <italic>RUNX2</italic>, <italic> SP7, AXIN2</italic> and <italic>DLX5</italic>, and the production of alkaline phosphatase and calcium. Coculture with MM cells failed to prevent osteogenic differentiation of adult human MSC. On the other hand, MM cells induced cell cycle progression in resting MSC in a cell contact dependent manner. This effect was confirmed using both primary CD138+ cells and MM cell lines, and was not seen with B or T cell lines. Our data confirm the presence of osteoblast progenitors and the preservation of osteogenic function in MM, however dysregulation of cell cycle control may contribute to the loss of normal bone homeostasis that ultimately results in osteolytic bone loss.</p> </abstract>