Cyclopamine did not affect mouse oocyte maturation in vitro but decreased early embryonic development. (3rd June 2014)

• Record Type: Journal Article
• Authors: Liu, Yang; Wei, Zhuying; Huang, Yafei; Bai, Chunling; Zan, Linsen; Li, Guangpeng
• Is Part Of: Animal science journal. Volume 85:Number 9(2014:Sep.)
• Page Start: 840
• Page End: 847
• Publication Date: 2014-06-03
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12220
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Hedgehog (Hh) pathway has been studied in various animal body life procedures and is suggested to be important for the development of multiple organs. The genes involved in the Hh signaling pathway were expressed in the ovary of mice, pigs and cattle. However, the function of Hh signaling pathway on oocyte maturation and early embryonic development is still controversial. We detected the effect of sonic hedgehog (Shh) and cyclopamine on the <italic>in vitro</italic> maturation of mouse oocytes and embryo development. The results showed that the presence of Shh or cyclopamine resulted in similar oocyte maturation to control groups. Shh did not improve early embryonic development. However, the supplement of cyclopamine depressed early embryo development. The mRNA of <italic>shh</italic>, <italic>ptch1</italic>, <italic>smo</italic> and <italic>gli1</italic> were less detected in the denuded oocytes. The expression levels of <italic>ptch1</italic> ascended from the uncleaved zygote to blastocyst stage. <italic>Smo</italic> or <italic>gli1</italic> were expressed on a higher level at the two‐cell or four‐cell stage in early embryonic development separately. Therefore, Shh did not affect mouse oocyte maturation and early embryo development, but cyclopamine led to inhibited development of mouse early embryo. The effects of Hh signaling on the oocyte maturation and early embryo development might be species‐specific.</p> </abstract>