In vivo imaging of bioluminescent Pseudomonas aeruginosa in an acute murine airway infection model. Issue 1 (30th June 2014)

• Record Type: Journal Article
• Authors: Munder, Antje; Wölbeling, Florian; Klockgether, Jens; Wiehlmann, Lutz; Tümmler, Burkhard
• Is Part Of: Pathogens and disease. Volume 72:Issue 1(2014:Oct.)
• Page Start: 74
• Page End: 77
• Publication Date: 2014-06-30
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12184
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12184-abs-0001"> <title>Abstract</title> <p>Non‐invasive bioluminescence imaging allows the analysis of infectious diseases in small animal models. In this study, an acute airway infection of C3H/HeN mice with <italic>luxCDABE</italic> transformed <italic>Pseudomonas aeruginosa</italic> TBCF10839 and an isogenic transposon mutant was followed by optical imaging <italic>in vivo</italic>. Using the disease‐causing dose of 2.0 × 10⁶ CFU of the cystic fibrosis airway isolate TBCF10839, subtle luminescence of the lungs was inconsistently visible for the first hour after infection. Conversely, using a 100‐fold higher dose of the strongly virulence‐attenuated transposon mutant, the robust signal of bioluminescent bacteria increased over 24 h. To monitor murine airway infections with <italic>P. aeruginosa in vivo</italic> by bioluminescence, one should select an attenuated mutant of a virulent strain or a wild type strain that naturally lacks virulence determinants and/or that has acquired a low virulence persister phenotype by patho‐adaptive mutations.</p> </abstract>