Selection of proper reference genes for the cyanobacterium Synechococcus PCC 7002 using real‐time quantitative PCR. Issue 1 (8th September 2014)

• Record Type: Journal Article
• Authors: Szekeres, Edina; Sicora, Cosmin; Dragoş, Nicolae; Drugă, Bogdan
• Is Part Of: FEMS microbiology letters. Volume 359:Issue 1(2014:Oct.)
• Page Start: 102
• Page End: 109
• Publication Date: 2014-09-08
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12574
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" id="fml12574-abs-0001"> <title>Abstract</title> <p> <italic>Synechococcus</italic> sp. PCC 7002 is known to be tolerant to most of the environmental factors in natural habitats of <italic>Cyanobacteria</italic>. Gene expression can be easily studied in this cyanobacterium, as its complete genome sequence is available. These properties make <italic>Synechococcus</italic> sp. PCC 7002 an appropriate model organism for biotechnological applications. To study the gene expression in <italic>Cyanobacteria</italic>, real‐time quantitative PCR (qPCR) can be used, but as this is a highly sensitive method, data standardization is indicated between samples. The most commonly used strategy is normalization against internal reference genes. <italic>Synechococcus</italic> sp. PCC 7002 has not yet been evaluated for the best reference genes. In this work, six candidate genes were analyzed for this purpose. Cyanobacterial cultures were exposed to several stress conditions, and three different algorithms were used for ranking the reference genes: geNorm, NormFinder, and BestKeeper. Moreover, gene expression stability value M and single‐control normalization error <italic>E</italic> were calculated. Our data provided a list of reference genes that can be used in qPCR experiments in <italic>Synechococcus</italic> sp. PCC 7002.</p> </abstract>