Characterization of recombinant human bone morphogenetic protein‐2 delivery from injectable hyaluronan‐based hydrogels by means of 125I‐radiolabelling. (25th August 2012)

• Record Type: Journal Article
• Authors: Piskounova, Sonya; Gedda, Lars; Hulsart‐Billström, Gry; Hilborn, Jöns; Bowden, Tim
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 8:Number 10(2014:Nov.)
• Page Start: 821
• Page End: 830
• Publication Date: 2012-08-25
• Subjects: Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.1584
• Languages: English
• ISSNs: 1932-6254

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>This study presents a thorough <italic>in vitro</italic> and <italic>in vivo</italic> characterization of the delivery of bone morphogenetic protein 2 (BMP‐2) from a hyaluronan‐based hydrogel system. The <italic>in vitro</italic> release of BMP‐2 from similar hydrogels has previously been studied by enzyme‐linked immunosorbent assay (ELISA), by which only a fraction of the loaded protein is detected. In the current study, <sup>125</sup>I radiolabelling was used instead to monitor BMP‐2 <italic>in vitro</italic> and <italic>in vivo</italic>. To minimize protein loss during handling, <sup>125</sup>I‐BMP‐2 adsorption to different tubes was studied at different times and temperatures. The data showed that Protein LoBind tubes exhibited the lowest protein affinity. Furthermore, a biphasic release profile of biologically active BMP‐2 was observed both <italic>in vitro</italic> and <italic>in vivo</italic>, with the initial fast phase during the first week, followed by a slower release during the remaining 3 weeks. The initial fast‐release phase corresponded to the early bone formation observed after 8 days in an ectopic model in rats. Bone volume and mineral content increased until day 14, after which a decrease in bone volume was observed, possibly due to resorption in response to decreased amounts of released BMP‐2. Overall, the results suggested that cautious protein handling and a reliable quantification technique are essential factors for successful design of a BMP‐2 delivery system. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>