Physico-chemical characterisation, cytotoxic activity, and biocompatibility studies of tamoxifen-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification method. (September 2014)

• Record Type: Journal Article
• Authors: Lakkadwala, Sushant; Nguyen, Sanko; Lawrence, Joseph; Nauli, Surya M.; Nesamony, Jerry
• Is Part Of: Journal of microencapsulation. Volume 31:Number 6(2014:Sep.)
• Page Start: 590
• Page End: 599
• Publication Date: 2014-09
• Subjects: Microencapsulation -- Periodicals; Controlled release technology -- Periodicals; 615.19
• DOI: 10.3109/02652048.2014.898707
• Languages: English
• ISSNs: 0265-2048

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Solid lipid nanoparticles (SLNs) can efficiently and efficaciously incorporate anti-cancer agents. <italic>Objective</italic>: To prepare and characterise tamoxifen (TAM)-loaded SLNs. <italic>Materials and methods</italic>: Glyceryl monostearate, Tween-80, and trehalose were used in SLNs. SLNs were tested via dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). <italic>Results</italic>: Characterisation studies revealed SLNs of about 540 nm with a negative surface charge and confirmed the entrapment of TAM in the SLNs. The entrapment efficiency was estimated to be 60%. <italic>Discussion</italic>: The <italic>in vitro</italic> drug release profile demonstrated a gradual increase followed by a release plateau for several days. A drug concentration-dependent increase in cytotoxic activity was observed when the SLNs were evaluated in cell cultures. <italic>Conclusion</italic>: Biocompatible and stable lyophilised SLNs were successfully prepared and found to possess properties that may be utilised in an anti-cancer drug delivery system.</p> </abstract>