Differential expression of poliovirus receptor, regulator of G-protein signaling 11 and erythrocyte protein band 4.1-like 3 in human granulosa cells during follicular growth and maturation. (September 2014)

• Record Type: Journal Article
• Authors: Barzilay, Eran; Yung, Yuval; Shapira, Lev; Haas, Jigal; Ophir, Libby; Yerushalmi, Gil M.; Maman, Ettie; Hourvitz, Ariel
• Is Part Of: Gynecological endocrinology. Volume 30:Number 9(2014:Sep.)
• Page Start: 660
• Page End: 663
• Publication Date: 2014-09
• Subjects: Endocrine gynecology -- Periodicals; Generative organs, Female -- Diseases -- Periodicals; 618.1
• DOI: 10.3109/09513590.2014.912268
• Languages: English
• ISSNs: 0951-3590

Abstract

<abstract> <title>Abstract</title> <p>Poliovirus receptor (<italic>PVR</italic>), regulator of G-protein signaling-11 (<italic>RGS11</italic>), and erythrocyte protein band-4.1-like 3 (<italic>EPB41L3</italic>) have been proposed to function in follicular maturation in mouse models. We have examined their expression in human mural (mGCs) and cumulus granulosa cells (CCs). Expression of <italic>PVR</italic> and <italic>RGS11</italic> in mGCs decreased in medium-sized follicles compared to small follicles of IVM cycles and increased again in large follicles. Luteinization caused decreased expression of both <italic>PVR</italic> and <italic>RGS11. In vitro</italic> incubation of mGCs with progesterone-rich conditioned media decreased expression of <italic>RGS11</italic> without affecting <italic>PVR</italic> levels. Inhibition of progesterone signaling enhanced expression of both <italic>RGS11</italic> and <italic>PVR</italic>. Expression in CCs was examined by means of global transcriptome sequencing analysis <italic>RGS11</italic> and <italic>EPB41L3</italic> increased in CCs during follicular maturation while <italic>PVR</italic> levels did not change. In conclusion, during human follicular maturation there are significant changes in expression of <italic>PVR, RGS11</italic> and <italic>EPB41L3</italic>, possibly regulated by progesterone.</p> </abstract>