SNP markers‐based map construction and genome‐wide linkage analysis in Brassica napus. Issue 7 (3rd April 2014)

• Record Type: Journal Article
• Authors: Raman, Harsh; Dalton‐Morgan, Jessica; Diffey, Simon; Raman, Rosy; Alamery, Salman; Edwards, David; Batley, Jacqueline
• Is Part Of: Plant biotechnology journal. Volume 12:Issue 7(2014:Sep.)
• Page Start: 851
• Page End: 860
• Publication Date: 2014-04-03
• Subjects: Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12186
• Languages: English
• ISSNs: 1467-7644

Abstract

<abstract abstract-type="main" id="pbi12186-abs-0001"> <title>Summary</title> <p>An Illumina Infinium array comprising 5306 single nucleotide polymorphism (SNP) markers was used to genotype 175 individuals of a doubled haploid population derived from a cross between Skipton and Ag‐Spectrum, two Australian cultivars of rapeseed (<italic>Brassica napus</italic> L.). A genetic linkage map based on 613 SNP and 228 non‐SNP (DArT, SSR, SRAP and candidate gene markers) covering 2514.8 cM was constructed and further utilized to identify loci associated with flowering time and resistance to blackleg, a disease caused by the fungus <italic>Leptosphaeria maculans</italic>. Comparison between genetic map positions of SNP markers and the sequenced <italic>Brassica rapa</italic> (A) and <italic>Brassica oleracea</italic> (C) genome scaffolds showed several genomic rearrangements in the <italic>B. napus</italic> genome. A major locus controlling resistance to <italic>L. maculans</italic> was identified at both seedling and adult plant stages on chromosome A07. QTL analyses revealed that up to 40.2% of genetic variation for flowering time was accounted for by loci having quantitative effects. Comparative mapping showed <italic>Arabidopsis</italic> and <italic>Brassica</italic> flowering genes such as <italic>Phytochrome</italic> A/D, <italic>Flowering Locus C</italic> and agamous‐Like MADS box gene <italic>AGL1</italic> map within marker intervals associated with flowering time in a DH population from Skipton/Ag‐Spectrum. Genomic regions associated with flowering time and resistance to <italic>L. maculans</italic> had several SNP markers mapped within 10 cM. Our results suggest that SNP markers will be suitable for various applications such as trait introgression, comparative mapping and high‐resolution mapping of loci in <italic>B. napus</italic>.</p> </abstract>