The 9‐lipoxygenase Osr9‐LOX1 interacts with the 13‐lipoxygenase‐mediated pathway to regulate resistance to chewing and piercing‐sucking herbivores in rice. Issue 1 (10th February 2014)

• Record Type: Journal Article
• Authors: Zhou, Guoxin; Ren, Nan; Qi, Jingfeng; Lu, Jing; Xiang, Caiyu; Ju, Hongping; Cheng, Jiaan; Lou, Yonggen
• Is Part Of: Physiologia plantarum. Volume 152:Issue 1(2014:Sep.)
• Page Start: 59
• Page End: 69
• Publication Date: 2014-02-10
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/ppl.12148
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main" id="ppl12148-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="ppl12148-para-0001">Oxylipins produced by the 13‐lipoxygenase (LOX) have been reported to play an important role in plant defense responses to herbivores. Yet, the role of oxylipins produced by the 9‐LOX pathway in this process remains largely unknown. Here we cloned a gene encoding a chloroplast‐localized 9‐LOX, <italic>Osr9‐LOX1</italic>, from rice. Transcriptional analysis revealed that herbivore infestation, mechanical wounding and jasmonic acid (JA) treatment either repressed or did not enhance the level of <italic>Osr9‐LOX1</italic> transcripts at early stages but did at later stages, whereas salicylic acid (SA) treatment quickly increased the transcript level of <italic>Osr9‐LOX1</italic>. Antisense expression of <italic>Osr9‐lox1</italic> (as‐<italic>r9lox1</italic>) decreased the amount of wound‐induced (<italic>Z</italic>)‐3‐hexenal but increased levels of striped stem borer (SSB)‐induced linolenic acid, JA, SA and trypsin protease inhibitors. These changes were associated with increased resistance in rice to the larvae of the SSB <italic>Chilo suppressalis</italic>. In contrast, although no significant differences were observed in the duration of the nymph stage or the number of eggs laid by female adults between the brown planthopper (BPH) <italic>Nilaparvata lugens</italic> that fed on as‐<italic>r9lox1</italic> lines and BPH that fed on wild‐type (WT) rice plants, the survival rate of BPH nymphs that fed on as‐<italic>r9lox1</italic> lines was higher than that of nymphs that fed on WT plants, possibly because of a higher JA level. The results demonstrate that Osr9‐LOX1 plays an important role in regulating an herbivore‐induced JA burst and cross‐talk between JA and SA, and in controlling resistance in rice to chewing and phloem‐feeding herbivores.</p> </abstract>