Cytokine gene expression profiles during initiation, progression and resolution of periodontitis. (24th July 2014)

• Record Type: Journal Article
• Authors: Ebersole, Jeffrey L.; Kirakodu, Sreenatha; Novak, Michael John; Stromberg, Arny J.; Shen, Shu; Orraca, Luis; Gonzalez‐Martinez, Janis; Burgos, Armando; Gonzalez, Octavio A.
• Is Part Of: Journal of clinical periodontology. Volume 41:Number 9(2014:Sep.)
• Page Start: 853
• Page End: 861
• Publication Date: 2014-07-24
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12286
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12286-abs-0001"> <title>Abstract</title> <sec id="jcpe12286-sec-0001" sec-type="section"> <title>Aim</title> <p>Variations in the expression of cytokines during the progression of periodontitis remain ill‐defined. We evaluated the expression of 19 cytokine genes related to T‐cell phenotype/function during initiation, progression and resolution of periodontitis, and related these to the expression of soft and bone tissue destruction genes (TDGs).</p> </sec> <sec id="jcpe12286-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>A ligature‐induced periodontitis model was used in rhesus monkeys (<italic>M. mulatta</italic>) (<italic>n</italic> = 18). Gingival tissues were taken at baseline pre‐ligation, 2 weeks and 1 month (Initiation) and 3 months (progression) post ligation. Ligatures were removed and samples taken 2 months later (resolution). Total RNA was isolated and the Rhesus Gene 1.0 ST (Affymetrix) used for gene expression analysis. Significant expression changes were validated by qRT‐PCR.</p> </sec> <sec id="jcpe12286-sec-0003" sec-type="section"> <title>Results</title> <p>Disease initiation/progression was characterized by overexpression of Th17/Treg cytokine genes (IL‐1<italic>β</italic>, IL‐6, TGF<italic>β</italic> and IL‐21) and down‐regulation of Th1/Th2 cytokine genes (IL‐18 and IL‐25). Increased IL‐2 and decreased IL‐10 levels were seen during disease resolution. Several Th17/Treg cytokine genes positively correlated with TDGs, whereas most Th1/Th2 genes exhibited a negative correlation.</p> </sec> <sec id="jcpe12286-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Initiation, progression and resolution of periodontitis involve over‐ and underexpression of cytokine genes related to various T‐helper subsets. In addition, variations in individual T‐helper response subset/genes during disease progression correlated with protective/destructive outcomes.</p> </sec> </abstract>