Sequence‐based identification, genotyping and EUCAST antifungal susceptibilities of Trichosporon clinical isolates from Greece. (22nd January 2014)

• Record Type: Journal Article
• Authors: Arabatzis, M.; Abel, P.; Kanellopoulou, M.; Adamou, D.; Alexandrou‐Athanasoulis, H.; Stathi, A.; Platsouka, E.; Milioni, A.; Pangalis, A.; Velegraki, A.; Lass‐Florl, C.
• Is Part Of: Clinical microbiology and infection. Volume 20:Number 8(2014:Aug.)
• Page Start: 777
• Page End: 783
• Publication Date: 2014-01-22
• Subjects: Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1111/1469-0691.12501
• Languages: English
• ISSNs: 1198-743X

Abstract

<abstract abstract-type="main" id="clm12501-abs-0001"> <title>Abstract</title> <p> <italic>Trichosporon</italic> yeasts constitute emerging pathogens, implicated in organ‐specific and systemic infections. In this first, comprehensive study of <italic>Trichosporon</italic> clinical isolates in Greece, 42 isolates were identified by sequencing the hypervariable D1/D2 domain of the Large Subunit (LSU) rDNA gene, while <italic>Trichosporon asahii</italic> were genotyped by sequencing the Intergenic Spacer 1 region, and antifungal susceptibilities were determined by the EDef 7.2 (EUCAST) method, in parallel with the CLSI standard. <italic>Trichosporon asahii</italic> was the primary species (37 isolates) followed by <italic>Trichosporon coremiiforme</italic>, <italic> Trichosporon dermatis</italic>, <italic> Trichosporon loubieri</italic> and <italic>Trichosporon mycotoxinivorans</italic>. One strain remained unidentified. Seven <italic>T. asahii</italic> genotypes were recorded. The major genotypes were: genotypes 4 (29%) and 3 (26%) followed by 1, 5 and 7 (9.5% each). Two novel genotypes were identified designated as 10 and 11. EUCAST MIC ≥2 mg/L was recorded in 58% of the isolates (amphotericin B), 41% (itraconazole), 41% (posaconazole) and 38% (voriconazole). Fluconazole MICs of ≥32 mg/L were recorded in 23.8% of the isolates. Analysis of variance performed on absolute values showed that the amphotericin B, itraconazole, posaconazole and voriconazole MICs of <italic>T. asahii</italic> were equivalent. Typically higher MIC values were displayed by fluconazole. Antifungal susceptibilities of the seven different genotypes were homogeneous. Agreements between EUCAST and CLSI ranged from 88.1 to 97.62%. Overall, the high MICs recorded among the <italic>Trichosporon</italic> isolates for all tested drugs justify routine susceptibility testing of clinical isolates.</p> </abstract>