ΑB‐Crystallin R120G variant causes cardiac arrhythmias and alterations in the expression of Ca2+‐handling proteins and endoplasmic reticulum stress in mice. (August 2014)

• Record Type: Journal Article
• Authors: Jiao, Qibin; Sanbe, Atsushi; Zhang, Xingwei; Liu, Jun‐Ping; Minamisawa, Susumu
• Is Part Of: Clinical and experimental pharmacology and physiology. Volume 41:Number 8(2014:Aug.)
• Page Start: 589
• Page End: 599
• Publication Date: 2014-08
• Subjects: Clinical pharmacology -- Periodicals; Pharmacology, Experimental -- Periodicals; Physiology, Experimental -- Periodicals; Physiology, Pathological -- Periodicals; 615.1
• DOI: 10.1111/1440-1681.12253
• Languages: English
• ISSNs: 0305-1870

Abstract

<abstract abstract-type="main" id="cep12253-abs-0001"> <title>Summary</title> <p>Mutations of <italic>α</italic>B‐crystallin (Cry<italic>α</italic>B), a small heat shock protein abundantly expressed in cardiac and skeletal muscles, are known to cause desmin‐related myopathies. The Cry<italic>α</italic>B R120G allele has been linked to a familial desminopathy and, in transgenic mice, causes a sudden death at about 28 weeks of age. To investigate the mechanisms of the sudden cardiac arrest of Cry<italic>α</italic>B R120G transgenic mice, we prepared protein samples from left ventricular tissues of two different age groups (10 and 28 weeks) and examined Ca<sup>2+</sup>‐handling proteins. Expression of sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) 2, phospholamban, ryanodine receptor 2 and calsequestrin 2 was significantly decreased in 28‐ versus 10‐week‐old Cry<italic>α</italic>B R120G transgenic mice. In addition, low heart rate variability, including heart rate, total power and low frequency, was observed and continuous electrocardiogram monitoring revealed cardiac arrhythmias, such as ventricular tachycardia, atrioventricular block and atrial flutter, in 28‐week‐old Cry<italic>α</italic>B R120G transgenic mice. In contrast, expression of endoplasmic reticulum (ER) degradation enhancing <italic>α</italic>‐mannosidase‐like protein, inositol requirement 1 and X‐box binding protein 1 were increased significantly in 28‐ versus 10‐week‐old Cry<italic>α</italic>BR120G transgenic mice, suggesting that the Cry<italic>α</italic>BR120G transgenic mice exhibit increased ER stress compared with wild‐type mice. Together, the data suggest that the Cry<italic>α</italic>B R120G dominant variant induces ER stress and impairs Ca<sup>2+</sup> regulation, leading to ageing‐related cardiac dysfunction, arrhythmias and decreased autonomic tone with shortened lifespan.</p> </abstract>