Liquid Chromatography–Mass Spectrometry and Chemometric Analysis of Ricinus communis Extracts for Cultivar Identification. Issue 5 (16th April 2014)

• Record Type: Journal Article
• Authors: Ovenden, Simon P. B.; Pigott, Eloise J.; Rochfort, Simone; Bourne, David J.
• Is Part Of: Phytochemical analysis. Volume 25:Issue 5(2014:Sep.)
• Page Start: 476
• Page End: 484
• Publication Date: 2014-04-16
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2519
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="pca2519-sec-0001" sec-type="section"> <title>Introduction</title> <p>Seeds of <italic>Ricinus communis</italic> contain the toxic protein ricin, a 64 kD heterodimeric type II ribosome‐inactivating protein that has been used in several high‐profile poisoning incidents. The ability to determine which cultivar the toxin was isolated from via an LC–MS method would be of significant use to law enforcement and forensic agencies.</p> </sec> <sec id="pca2519-sec-0002" sec-type="section"> <title>Objective</title> <p>To analyse via LC–MS and chemometrics (principal components analysis (PCA), orthogonal partial‐least‐squares discriminant analysis (OPLS‐DA)) extracts of <italic>R. communis</italic> to identify compounds specific to a particular cultivar.</p> </sec> <sec id="pca2519-sec-0003" sec-type="section"> <title>Methods</title> <p>Seeds from eight specimens of six cultivars of <italic>R. communis</italic> ('<italic>carmencita</italic>', '<italic>dehradun</italic>', '<italic>gibsonii</italic>', '<italic>impala</italic>', '<italic>sanguineus</italic>' and '<italic>zanzibariensis</italic>') were extracted using a standard methodology. These extracts were analysed by LC–MS then subjected to chemometric analysis (PCA and OPLS‐DA). Identified compounds of importance were subjected to high‐resolution Fourier transform (HRFT) MS and MS/MS to elucidate their structures.</p> </sec> <sec id="pca2519-sec-0004" sec-type="section"> <title>Results</title> <p>This analysis identified 17 ions as potential cultivar determinators. Through accurate mass measurement and MS/MS, molecular formulae for 13 ions were determined, including two known and 11 new peptides.</p> </sec> <sec id="pca2519-sec-0005" sec-type="section"> <title>Conclusion</title> <p>Unique ions in extracts of '<italic>carmencita</italic>', '<italic>dehradun</italic>', '<italic>gibsonii</italic>', '<italic>impala</italic>' and '<italic>zanzibariensis</italic>' were identified that would allow an individual cultivar to be distinguished from other cultivars in this study. Although '<italic>sanguineus</italic>' extracts contained no unique compounds, a unique LC–MS profile would allow for cultivar assignment. Copyright © 2014 John Wiley & Sons, Ltd.</p> </sec> </abstract>