A Simple Semi‐preparative Reversed‐phase HPLC/PDA Method for Separation and Quantification of Glycyrrhizin in Nine Samples of Glycyrrhiza glabra Root Collected from Different Geographical Origins. Issue 5 (3rd March 2014)

• Record Type: Journal Article
• Authors: Basar, Norazah; Talukdar, Anupam D.; Nahar, Lutfun; Stafford, Angela; Kushiev, Habibjon; Kan, Asuman; Sarker, Satyajit D.
• Is Part Of: Phytochemical analysis. Volume 25:Issue 5(2014:Sep.)
• Page Start: 399
• Page End: 404
• Publication Date: 2014-03-03
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2507
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="pca2507-sec-0001" sec-type="section"> <title>Introduction</title> <p> <italic>Glycyrrhiza glabra</italic> L. (Fabaceae), commonly known as 'liquorice', is one of the most popular ingredients in several traditional herbal medicinal preparations, and glycyrrhizin is the major glycoside present in this plant. The content of glycyrrhizin may vary among <italic>G. glabra</italic> samples collected from various geographical origins, which may affect the therapeutic efficacy. Thus, quantification of glycyrrhizin in <italic>G. glabra</italic> samples is important.</p> </sec> <sec id="pca2507-sec-0002" sec-type="section"> <title>Objective</title> <p>To develop and validate a simple semi‐preparative reversed‐phase HPLC with photodiode array (PDA) method for separation and quantification of glycyrrhizin in nine samples of <italic>G. glabra</italic> root collected from various geographical origins.</p> </sec> <sec id="pca2507-sec-0003" sec-type="section"> <title>Methods</title> <p>Dried and ground root of <italic>G. glabra</italic> was Soxhlet‐extracted sequentially with <italic>n</italic>‐hexane and methanol (MeOH). The separation and quantification of glycyrrhizin was achieved on a C<sub>18</sub> reversed‐phase semi‐preparative column using a gradient mobile phase, 30–100% solvent B in solvent A in 30 min (solvent A: 0.1% v/v trifluoroacetic acid (TFA) in water and solvent B: 0.1% v/v of TFA in MeOH), at a flow rate of 3.00 mL/min and UV detection at 254 nm.</p> </sec> <sec id="pca2507-sec-0004" sec-type="section"> <title>Results</title> <p>A simple semi‐preparative reversed‐phase HPLC/PDA method allowing clear separation and quantification of glycyrrhizin content in nine samples has been validated in terms of linearity, selectivity, limits of detection, precision, accuracy and detection. Concentration levels of glycyrrhizin were between 0.177 and 0.688% w/w of dry materials.</p> </sec> <sec id="pca2507-sec-0005" sec-type="section"> <title>Conclusion</title> <p>This method is precise, less time consuming and more cost effective, and can be used for the quality control of any <italic>G. glabra</italic> sample with regard to its glycyrrhizin contents. Copyright © 2014 John Wiley & Sons, Ltd.</p> </sec> </abstract>