Hydrogen sulfide treatment induces angiogenesis after cerebral ischemia. Issue 11 (17th June 2014)

• Record Type: Journal Article
• Authors: Jang, Hyunduk; Oh, Mi‐Young; Kim, Young‐Ju; Choi, In‐Young; Yang, Hye sung; Ryu, Wi‐Sun; Lee, Seung‐Hoon; Yoon, Byung‐Woo
• Is Part Of: Journal of neuroscience research. Volume 92:Issue 11(2014:Nov.)
• Page Start: 1520
• Page End: 1528
• Publication Date: 2014-06-17
• Subjects: Neurobiology -- Periodicals; 612
• DOI: 10.1002/jnr.23427
• Languages: English
• ISSNs: 0360-4012

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Hydrogen sulfide (H<sub>2</sub>S) is a potent vasodilator and regulates cardiovascular homeostasis. Furthermore, H<sub>2</sub>S has a crucial role in ischemia−reperfusion injuries, especially of the heart, liver, and kidneys. This study indicates that treatment with hydrogen sulfide is able to restore neurological function after ischemic stroke by promoting angiogenesis. Treatment with H<sub>2</sub>S augments angiogenesis in the peri‐infarct area, and it significantly improves functional outcomes after 2 weeks in a rat MCAO model. H<sub>2</sub>S promotes the phosphorylation of AKT and ERK and increases the expression of vascular endothelial growth factor (VEGF) and angiopoietin‐1 (Ang‐1). H<sub>2</sub>S‐treated rats showed more newly synthesized endothelial cells in the ischemic lesion (2.31‐fold, <italic>P</italic> < 0.01). H<sub>2</sub>S‐treated astrocytes increased VEGF and Ang‐1 expression, and the inhibition of phosphatidylinositide 3‐kinase (PI3K)/AKT signaling by LY294002 significantly reduced H<sub>2</sub>S‐induced VEGF and Ang‐1 expression in astrocytes. Finally, H<sub>2</sub>S stimulated endothelial cell migration (3.92‐fold increase in wound healing assay) and tube formation (3.69‐fold increase, <italic>P</italic> < 0.001) through PI3K/AKT signaling. In conclusion, treatment with H<sub>2</sub>S promotes angiogenesis and thereby contributes to improvement of functional outcome after cerebral ischemia. Our findings strongly suggest that H<sub>2</sub>S may be of value in regenerative recovery after stroke. © 2014 Wiley Periodicals, Inc.</p> </abstract>