Enhanced cardioprotective effects mediated by plasmid containing the short‐hairpin RNA of angiotensin converting enzyme with a biodegradable hydrogel after myocardial infarction. Issue 10 (12th November 2013)

• Record Type: Journal Article
• Authors: Wan, Wei‐Guo; Jiang, Xue‐Jun; Li, Xiao‐Yan; Zhang, Cui; Yi, Xin; Ren, Shan; Zhang, Xian‐Zheng
• Is Part Of: Journal of biomedical materials research. Volume 102:Issue 10(2014)
• Page Start: 3452
• Page End: 3458
• Publication Date: 2013-11-12
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35014
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The expression of foreign gene was enhanced and prolonged by sustained releasing a target gene to cells from biodegradable dextran‐poly(e‐caprolactone)‐2‐hydroxylethylmethacrylate‐poly(<italic>N</italic>‐isopropylacrylamide) (Dex‐PCL‐HEMA/PNIPAAm) hydrogel <italic>in vitro</italic>. Moreover, we have demonstrated that injection of the same hydrogel improved post‐infarct ventricular remodeling. Therefore, we hypothesized that intramyocardial injection of plasmid containing the short‐hairpin RNA (shRNA) of angiotensin converting enzyme (ACE) with the same hydrogel enhances the cardioprotective effects superior to either alone or after rat myocardial infarction (MI). In this study, equal volume of phosphate‐buffered solution (PBS), 10 μg ACE‐shRNA plasmids, hydrogel containing 10 μg negative control ACE‐shRNA plasmids and hydrogel containing 10 μg ACE‐shRNA plasmids were shortly injected into the infarct area of rats after MI, respectively. We found that ACE‐shRNA plasmid‐loaded hydrogel extended the duration of gene expression <italic>in vivo</italic>. Moreover, it was shown that direct intramyocardial injection of ACE‐shRNA plasmid‐loaded hydrogel significantly decreased the expression of local ACE expression, inhibited cell apoptosis, reduced infarct size, and improved cardiac function compared with the injection of either alone 30 days after MI in rats. These results suggest that injection of ACE‐shRNA plasmid‐loaded hydrogel into impaired myocardium obtains more cardioprotective effects than either alone in rat with MI by prolonging the gene silencing of ACE. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3452–3458, 2014.</p> </abstract>