Bacterial‐induced expression of RAB18 protein in Orzya sativa salinity stress and insights into molecular interaction with GTP ligand. Issue 9 (24th June 2014)

• Record Type: Journal Article
• Authors: Jha, Yachana; Sablok, Gaurav; Subbarao, Naidu; Sudhakar, Raja; Fazil, M. H. U. Turabe; Subramanian, R. B.; Squartini, Andrea; Kumar, Sunil
• Is Part Of: Journal of molecular recognition. Volume 27:Issue 9(2014:Sep.)
• Page Start: 521
• Page End: 527
• Publication Date: 2014-06-24
• Subjects: Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2371
• Languages: English
• ISSNs: 0952-3499

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>In the present research, we have studied the inoculation effects of two root‐associated plant growth‐promoting rhizobacteria (PGPR) in rice and provide the pieces of evidence that the inoculation of the PGPR could potentially result in inducing the expression of the salt stress‐related <italic>RAB18</italic> plant gene under varying degrees of salinity stress. The sequenced putative gene of <italic>RAB18</italic> of <italic>Oryza sativa</italic> in this study is 740 bp long, has a content of 44.4%, and a molecular weight of 492 102.00 Da. BLAST homology patterns revealed sequence similarity with the previously sequenced <italic>RAB</italic> in model plant species. We demonstrate the mode of action of this stress‐related protein by performing comparative modeling of <italic>Q10RT8 (Os03g0146000</italic> protein, homolog of the sequenced <italic>RAB18; O</italic>. <italic>sativa</italic> subsp<italic>. japonica)</italic> using energy minimization, molecular dynamic simulations, and molecular docking of a guanosine triphosphate (<italic>GTP</italic>) ligand with the protein. The docking results indicated that <italic>Ser</italic>21, <italic>Ala</italic>22, <italic>Lys</italic>25, <italic>Asp</italic>68, <italic>Ala</italic>70, <italic>Glu</italic>73, and <italic>Arg</italic>74 are important determinant residues for functional interaction with the <italic>GTP</italic> ligand. The present research contributes to the understanding of the PGPR inoculation in salinity stress. Additionally, it provides the layout of the understanding of the molecular interactions between <italic>RAB</italic> and <italic>GTP</italic> ligand. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>