TLR ligands of ryegrass pollen microbial contaminants enhance Th1 and Th2 responses and decrease induction of Foxp3hi regulatory T cells. Issue 3 (18th January 2013)

• Record Type: Journal Article
• Authors: Mittag, Diana; Varese, Nirupama; Scholzen, Anja; Mansell, Ashley; Barker, Gillian; Rice, Gregory; Rolland, Jennifer M.; O'Hehir, Robyn E.
• Is Part Of: European journal of immunology. Volume 43:Issue 3(2013:Mar.)
• Page Start: 723
• Page End: 733
• Publication Date: 2013-01-18
• Subjects: Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201242747
• Languages: English
• ISSNs: 0014-2980

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Microbial contamination of grass pollens could affect sensitization, subsequent allergic response, and efficacy of allergen‐specific immunotherapy. We investigated whether bacterial immunomodulatory substances can direct PBMC responses of allergic and nonatopic subjects against ryegrass pollen (RGP) toward Th1, Th2, or regulatory T (Treg) cells. Aqueous extracts of RGP with high or low LPS were fractionated into large and small molecular weight (MW) components by diafiltration. CFSE‐labeled PBMCs from allergic and nonatopic subjects were stimulated with RGP extracts (RGPEs) and analyzed for cytokine secretion and T‐cell responses. High LPS RGPE increased IFN‐γ<sup>+</sup> Th1 and IL‐4<sup>+</sup> Th2 effector cell induction and consistently decreased CD4<sup>+</sup>Foxp3<sup>hi</sup> Treg‐cell induction. IL‐10‐producing T‐cell frequency was unaltered, but IL‐10 secretion was increased by high LPS RGPE. RGPE‐stimulation of TLR‐transfected cell lines revealed that high LPS pollen also contained a TLR2‐ligand, and both batches a TLR9‐ligand. Beta‐1, 3‐glucans were detected in large and small MW fractions and were also T‐cell stimulatory. In conclusion, coexposure to allergen and proinflammatory microbial stimuli does not convert an established Th2‐ into a Th1‐response. Instead, proinflammatory responses are exacerbated and Foxp3<sup>hi</sup> Treg‐cell induction is decreased. These findings show that adjuvants for specific immunotherapy should enhance Treg cells rather than target immune deviation from Th2 to Th1.</p> </abstract>