Butyrate production in engineered Escherichia coli with synthetic scaffolds. Issue 10 (22nd April 2013)

• Record Type: Journal Article
• Authors: Baek, Jang‐Mi; Mazumdar, Suman; Lee, Sang‐Woo; Jung, Moo‐Young; Lim, Jae‐Hyung; Seo, Sang‐Woo; Jung, Gyoo‐Yeol; Oh, Min‐Kyu
• Is Part Of: Biotechnology and bioengineering. Volume 110:Issue 10(2013:Oct.)
• Page Start: 2790
• Page End: 2794
• Publication Date: 2013-04-22
• Subjects: Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.24925
• Languages: English
• ISSNs: 0006-3592

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="bit24925-sec-0001" sec-type="section"> <p>Butyrate pathway was constructed in recombinant <italic>Escherichia coli</italic> using the genes from <italic>Clostridium acetobutylicum</italic> and <italic>Treponema denticola</italic>. However, the pathway constructed from exogenous enzymes did not efficiently convert carbon flux to butyrate. Three steps of the productivity enhancement were attempted in this study. First, pathway engineering to delete metabolic pathways to by‐products successfully improved the butyrate production. Second, synthetic scaffold protein that spatially co‐localizes enzymes was introduced to improve the efficiency of the heterologous pathway enzymes, resulting in threefold improvement in butyrate production. Finally, further optimizations of inducer concentrations and pH adjustment were tried. The final titer of butyrate was 4.3 and 7.2 g/L under batch and fed‐batch cultivation, respectively. This study demonstrated the importance of synthetic scaffold protein as a useful tool for optimization of heterologous butyrate pathway in <italic>E. coli</italic>. Biotechnol. Bioeng. 2013;110: 2790–2794. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>